In vitro shoot and root proliferation of jack fruit as affected by different concentrations of growth regulators.

The research work relating to the micro-propagation of Jack fruit was carried out in the tissue culture lab of the Horticultural Research Institute, NARC, Islamabad during the year 2004 with the objective of finding a suitable protocol for its mass multiplication. Explants grown from seedlings in the greenhouse were surface sterilized with NaOCl. For this purpose, four levels of NaOCl i.e., 0.5, 0.75, 1.0, 1.25 % w/v were used. MS basal medium containing different concentrations of BAP (1.0, 1.5, 2.0 and 2.5 mg l) was used for shoot proliferation. Different concentrations of IBA (1.0, 2.0, 3.0, 4.0, and 5.0 mg l) were used for determining the in-vitro rooting potential. The best survival was observed with 0.75% NaOCl which gave maximum survival (46.66 %) while BAP @ 1.5 mg l exhibited the highest number of shoots. Higher rooting rate (%) and number of roots per plant were obtained with IBA @ 3.0 mg l.