The Nse3/MAGE and Nse4/EID proteins are involved intranscription regulation
2010
The SMC5/6 complex is composed of two subcomplexes
SMC5-SMC6-Nse2 and Nse1-Nse3-Nse4. Nse3 shows sequence
similarity to the MAGE (Melanoma Antigen Gene) family of
proteins, which are overexpressed in certain types of cancers
and whose function has been linked to cell cycle regulation,
apoptosis and neuronal development. Using site-directed
mutagenesis, protein-protein interaction analyses and modelling
of the Schizosaccharomyces pombe Nse3 onto the crystal
structure of the MAGEA4 protein, we have identified surfaces on
the C- and N-terminal domains of Nse3 that interact
respectively with Nse4 and Nse1. The binding site for Nse4 is
evolutionarily conserved within the Nse3/MAGE superfamily of
proteins. Nse4 is related to the EID (E1A-like inhibitor of
differentiation) family of transcriptional repressors. We show
that there is interaction between members of the MAGE family
and those of the EID family. Our data suggest that the
Nse3/MAGE and Nse4/EID proteins are involved in gene
regulation.
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