Development of multicolour real time PCR for detection of drug resistance mutation in hepatitis B virus

Objective To set up a rapid method for detection of drug resistance mutation in HBV,based on multicolour real time PCR. To detect the mutation of blood serum, which were collected from patients. Method To establish two reaction systems, each reaction system contains four resistance loci. On the new multicolor real time PCR method, the sensitivity and specificity were analysed, and detecting the drug resistant mutation of blood serum collected from 30 cases patients. Results Construction of a multicolor fluorescence PCR for detection drug resistance in HBV was constructed, better specificity,sensitivity analysis of up to 1 × 103 copies/ml. Sample of 30 cases were detected, there were 2 YVDD (6.67%), 1 YIDD (3.33%), and there were 5 cases of 1896 variation, accounting for 16. 67%. Other sites were not detected mutations. Conclusion The multicolor real time PCR detection system could be used for rapid and simple analysis of drug resistance for the clinical hospital. The 1986 mutation in HBV pre-C region are relatively high. Key words: Polymerese chain reaction;  Hepatitis B virus;  Drug resistance, viral;  Mutation