Chemical constituents and bioactivities of Garcinia malaccensis Hk.f

Garcinia malaccensis Hk.f (Guttiferae) is closely resembled to G. mangostana and G. hombroniana. The genus of Garcinia, especially Garcinia mangostana is well known as a medicinal plant in South-east Asia and is used in traditional medicine. The paper reported the chemical constituents of the stembark of Garcinia malaccensis together with cytotoxic, antimicrobial and antioxidant activities. Purification and structure elucidation were carried out by chromatographic and spectroscopic techniques, respectively. MTT and trypan blue exclusion methods were performed to study the cytotoxic activity. Antibacterial activity was conducted by disc diffusion and microdilution methods, whereas antioxidant activities were done by ferric thiocyanate method and DPPH radical scavenging. The phytochemical study led to the isolation of -mangostin and cycloart-24-en-3-ol. -Mangostin exhibited cytotoxic activity against HSC-3 cells with an IC50 of 0.33 M. β- and α-mangostin showed activity against K562 cells with IC50 of 0.40 M and 0.48 M, respectively. -Mangostin was active against Gram-positive bacteria, Staphylococcus aureus and Bacilus anthracis with inhibition zone and MIC value of (19 mm; 0.025 mg/mL) and (20 mm; 0.013 mg/mL), respectively. In antioxidant assay, -mangostin exhibited activity as an inhibitor of lipid peroxidation. G. malaccensis presence - and -mangostin and cycloart-24-en-3-ol. -Mangostin was found very active against HSC-3 cells and K562. The results suggest that mangostins derivatives have the potential to inhibit the growth of cancer cells by inducing apoptosis. In addition, -and -mangostin was found inhibit the growth of Gram-positive pathogenic bacteria and also showed the activity as an inhibitor of lipid peroxidation. Keywords: Garcinia malaccensis, Guttiferae, apoptosis, antibacterial, antioxidant