Effect of Dahuangzhechong Pill on the Gene Expression Spectra of Preventing Arterial Thrombosis

Objective:To investigate the effect of Dahuangzhechong pill on the gene expression spectra of preventing arterial thrombosis,and reveal its mechanism on molecule level.Methods:Mononuclear cell and blood platelet of the arterial thrombosis patients were separated before and after treatment by Dahuangzhechong pill.Their RNA was extracted respectively and the genes expressions were detected using gene array containing 14 000 gene.Results:44 genes up-expressed and 299 genes down-expressed in blood platelet,252 genes expression increased and 299 genes expression decreased in mononuclear cell genes after treated with Dahuangzhechong pill.The cluster analysis showed that the genes contained ion channel and transport protein,apoptosis related protein,DNA synthesis,repair and transcriptio factor,cell receptor,cell signal and transducin,and protein translation and synthesis,etc.Conclusion:Dahuangzhechong pill may prevent arterial thrombosis through genes containing ion channel and transport protein,apoptosis related protein,DNA synthesis,repair and transcriptio factor,cell receptor,cell signal and transducin,and protein translation and synthesis,etc.