The Promoting Effect of Dansam-Samultang on Hair Growth by Gross and Histological Observation

The purpose of this study was to evaluate the hair growth effect of Dansam-Samultang in C57BL/6 mice. In order to estimate its hair growth effect, we performed percutaneous application of sample on the back of six-week-old C57BL/6 male mice; 150 μL a day, six days a week, for four weeks. Experimental animals were classified into four groups control group (C, saline), positive control group (PC, minoxidil 3%), experimental 1 group (E1, Dansam-Samultang ethanol 2%), and experimental 2 group (E2, Dansam-Samultang toner 2%). Body weight, water and feed consumption were measured once a week, and macroscopic hair growth effect was observed once a week. On the second and fourth week, we took a blood sample of five mice, analyzed their blood cells, observed their skin tissues. The experiment showed no significance in water and feed consumption, body weight gain, and feed efficiency among groups; relatively high weight gain, but no statistical significance for PC group. The organ weight had no significant difference among groups. In the hematological analysis, E1 had more white blood cells, neutrophils, and monocytes, but there was no significant difference in other items among groups. Macroscopic observation showed hair growth effect in the high to low order of PC, E1, E2, and C groups. Histological observation showed a clear increase of number and depth of follicle, and dermal thickness for PC, E1 and E2 groups, compared to C group. Taking all the results together, this experiment shows that Dansam-Samultang has a effective in hair growth and hair loss prevention.