Chemical constituents and bioactivity of Teucrium pilosum

Objective: To study the chemical constituents and bioactivity of Teurium pilosum.Method: Various column chromatographic techniques were used to isolate the constituents.A combination of EI-MS,NMR spectroscopy and X-Ray were applied to identify the structures.The anti-microorganism was accomplished by disk diffusion method,the antioxidant activity was assayed by the DPPH microanalysis models and the inhibitory activity of α-glucosidase was screened In vitro.Result: Eight compounds were isolated and identified as: glyceryl tristearate(1),2,5-dioxolanone(2),fernenol(3),stigmasta-5,22-dien-3β-ol(4),24-nor cholesta-5,22(E)-dien-3β-ol(5),α-spinasterol(6),(3,4-dihydroxyphenyl) acrylate(7),3,4-dihydroxy phenyl acrylic acid(8).Conclusion: All compounds have been isolated from the genus for the first time.Compound 3 [IC50=(37.63±3.45) mg·L-1],6 [IC50=(178.92±4.99) mg·L-1] and 8 [IC50=(44.32±7.02) mg·L-1] are of higher inhibitory α-glucosidase activity than that of acarbose [C50=(1 081.27±12.3) mg·L-1].Compound 7 [IC50=(4.81±0.96) mg·L-1] and 8 [IC50=(4.16±0.11) mg·L-1] showed higher antioxidant activity than that of BHT [IC50=(35.64±0.36) mg·L-1] and BHA [IC50=(8.74±0.39) mg·L-1].Compound 5-8 exhibited inhibitory activity against Fusarium graminearum.Compound 5 and 8 showed inhibitory activity against Botrytis cinerea.