Possible selection bias limits the interpretation of single-cell transcriptomics data of steroid-resistant asthma exacerbation

We believe there are a few shortcomings in the study design and data interpretation in the research article by Wang et al. (1). These include 1) cell selection bias, 2) cytokine selection bias from single-cell transcriptomics (SCT) data, and 3) lack of a lipopolysaccharide+dexamethasone (LPS+DEX) control group in the mouse model of steroid-resistant asthma (SRA) exacerbation. In figure 3C of ref. 1 the authors have selected five cell clusters including macrophages and group 2 innate lymphoid cells (ILC2) from among 20 cell types. This seems to be incorrect for several reasons. First, the authors have not chosen neutrophils, which play a key role in the pathogenesis of SRA (2). It is also unclear why they did not find … [↵][1] 1To whom correspondence may be addressed. Email: mabsome{at}yahoo.co.in. [1]: #xref-corresp-1-1