Medial Nucleus Accumbens Projections to the Ventral Tegmental Area Control Food Consumption.

Decades of research have shown that the nucleus accumbens (NAc) is a critical region influencing addiction, mood, and food consumption through its effects on reinforcement learning, motivation, and hedonic experience. Pharmacological studies have demonstrated that inhibition of the NAc shell induces voracious feeding, leading to the hypothesis that the inhibitory projections that emerge from the NAc normally act to restrict feeding. While much of this work has focused on projections to the lateral hypothalamus (LH), the role of NAc projections to the ventral tegmental area (VTA) in the control food intake has been largely unexplored. Using a retrograde viral labeling technique and real time monitoring of neural activity with fiber photometry, we find that medial NAc shell projections to the VTA (mNAccVTA) are inhibited during food-seeking and food consumption in male mice. We also demonstrate that this circuit bidirectionally controls feeding: optogenetic activation of NAc projections to the VTA inhibits food seeking and food intake (in both sexes), while optogenetic inhibition of this circuit potentiates food seeking behavior. Additionally, we show that activity of the NAc to VTA pathway is necessary for adaptive inhibition of food intake in response to external cues. These data provide new insight into NAc control over feeding in mice, and contribute to an emerging literature elucidating the role of inhibitory midbrain feedback within the mesolimbic circuit. Significance Statement The medial nucleus accumbens has long been known to control consummatory behavior, with particular focus on accumbens projections to the lateral hypothalamus. Conversely, NAc projections to the VTA have mainly been studied in the context of drug reward. We show that NAc projections to the VTA bidirectionally control food intake, consistent with a permissive role in feeding. Additionally, we show that this circuit is normally inactivated during consumption and food-seeking. Together, these findings elucidate how mesolimbic circuits control food consumption.