Evaluation of antioxidant response and Na+-K+-ATPase activity in zebrafish exposed to polyethylene microplastics: Shedding light on a physiological adaptation

Abstract Although the toxicity of microplastics has already been demonstrated in different animal models, our knowledge about the response of freshwater fish to this pollutant is still limited. Thus, we aimed to evaluate the impact of exposure of zebrafish adults (Danio rerio) to environmentally relevant concentrations of polyethylene microplastics (PE-MPs) (5 and 50 µg/L) and at different times of exposure (10 and 20 days). Initially, scanning electron microscope image illustrated size and format of the particle and FTIR analysis confirmed the presence of PE-MPs in the gastrointestinal tract of fish (at both concentrations tested). Subsequently, an alteration of oxidative and antioxidant responses was evaluated in the liver and brain. The results showed that catalase (CAT) activity, in liver, was significantly decreased, as was glutathione S-transferases (GSTs) activity (on the 10th experimental day). However, after 20 days of exposure, we observed a concentration-dependent increase in GST activity in liver of the animals exposed to PE-MPs. Furthermore, the lipid peroxidation (LPO) level was significantly increased by exposure to MPs, especially in the brain, after 20 days of exposure. The increase in Na+-K+-ATPase activity in the animals' gills was correlated with the increased production of malondialdehyde (MDA), which suggests the existence of a compensatory mechanism in which the high activity of this enzyme would be necessary to regulate the loss of ions caused by the increase in the processes of LPO, which has never been previously demonstrated. Thus, our study sheds light on a new physiological adaptation to deal with the oxidative effects of PE-MPs, in addition to supporting the future use of the assessment of Na+/K+-ATPase activity as a biomarker of the toxicity of these pollutants.