Determination of log D via automated microfluidic liquid-liquid extraction.

A method for log D (pH 7.4) measurement was developed using microfluidic liquid−liquid extraction. Values were determined for 26 compounds and compared to results obtained via shake-flask methods. Excellent correlation between the values obtained via both methods was achieved (R2 = 0.994). The developed methodology is amenable to automation, enabling high-throughput determination of large compound collections.