Preparation method for pathogenic yersinia enterocolitica test strips

The invention relates to a preparation method for pathogenic yersinia enterocolitica test strips, effectively solving the problems of high time consumption, complexity and poor specificity of existing detection methods. The preparation method includes adding n-caprylic acid to mouse ascites and acetate buffer solution, mixing evenly and centrifuging the mixture, regulating the pH (potential of hydrogen) value of supernatant, adding ammonium sulfate, performing centrifugation, dissolving sediments by PBS solution, and performing dialysis to obtain monoclonal antibodies; activating red, blue and yellow latex particles with carboxyl groups by carbodiimide respectively to obtain suspended activated latex particles; dialyzing to-be-marked antibodies, adding the monoclonal antibodies, dropping the mixture into the activated latex particles, rinsing with NaH2PO4 to enable sediments to be suspended in bovine serum albumin buffer solution, adsorbing markers by glass fibers, drying the markers, and curing anti-mouse IgG (immunoglobulin G) antibodies on fiber films to form quality control areas. The preparation method has the advantages that the advantages of immunoreaction specificity, chromatography rapidity and intuition of the colored latex particles serving as tracers are combined, and accordingly, simplicity, convenience, specificity and sensitiveness are achieved.