Mutant of HPV33 (human papilloma virus 33) L1 protein

The invention relates to mutational HPV33 (human papilloma virus 33) L1 protein (or a mutant thereof), an encoding sequence and a preparation method thereof, and a virus-like particle containing the same. The protein (or the mutant thereof) and the virus-like particle can be used for inducing a neutralizing antibody for resisting at least two types of HPV (such as HPV33 and HPV58, or HPV33, HPV58 and HPV52), so as to prevent the infection of at least two types of HPV and the diseases caused by infection, such as cervical cancer and condyloma acuminatum. The invention also relates to application of the protein and the virus-like particle in preparing a medicine composition or a vaccine. The medicine composition or the vaccine can be used for preventing the infection of at least two types of HPV and the diseases caused by infection, such as cervical cancer and condyloma acuminatum.