Rapid PLBs Proliferation of Cymbidium spp. In vitro

Optimum media salts concentration is essential for in vitro rapid propagation of orchid to provide sufficient nutrient require to promote metabolism and cell growth and to prevent toxic effect of media salts. Trehalose has a strong stabilizing effect on biological structures, forming a glass-like structure after dehydration. Because of these characteristics, trehalose is predicted to become a useful stabilizer in foods and an additive in cosmetics and pharmaceuticals. The aim of this study is to integrate findings on the regulatory function of hyaluronic acid (HA) with trehalose to effects on growth and development of protocorm-like bodies (PLB) of two Cymbidium spp. Results of this study revealed that low concentration of HA with trehalose in modified MS medium increased maximum formation of PLB within short duration of culture. In Cymbidium insigne, 100% PLB formation rate was observed on medium containing trehalose with 0.1 mg/l HA9 or 1 mg/l HA9 after 5 weeks of culture. In Cymbidium dayanum, 80% PLB formation rate was observed on media containing trehalose with 0.01 mg/l HA9 after 3 weeks of culture. Based on our present study we concluded that trehalose and hyaluronic acid added with culture medium, rapidly increased the formation rate of PLB, shoot and root in vitro culture of Cymbidium spp.