Ação do plasma rico em plaquetas autólogo em autoenxertos de pele em equinos

EnglishThis study evaluated the action of autologous platelet-rich plasma (PRP) on cutaneous wounds, containing skin autografts, in the gluteal region of horses. Seven healthy horses were used. Two 6 x 6cm cutaneous wounds were produced on each side of the gluteal region. Eight days after wound induction, grafts were performed with skin fragments harvested from the neck, as well as the application of PRP, prepared by double-centrifugation protocol. Wounds with autografts on the left side received PRP (group T), and those with autografts on the right side did not receive treatment (group C). Macroscopic and microscopic evaluations were performed, considering the integration of autografts and retraction of wound edges, as well as neovascularization, inflammatory infiltrate, young fibroblasts, collagenization, reepithelization and autografts integration. There was no difference between the groups (P > 0.05) in relation to most macroscopic and microscopic variables. However, neovascularization was significantly greater (p = 0.0191) in group T, on the 14th day after grafting. It is concluded that PRP favors the process of skin repair with autografts in horses, since it increases the neovascularization in the initial phase of wound healing. Furthermore, the PRP seems to positively influence the integration of the skin autografts and the retraction of the wound edges. portuguesEste estudo avaliou a acao do plasma rico em plaquetas (PRP) autologo em feridas cutâneas, contendo autoenxertos de pele em equinos. Foram utilizados sete equinos higidos, nos quais foram produzidas duas feridas cutâneas de 6 x 6cm, em cada um dos lados da regiao glutea. Oito dias apos a inducao das feridas, foram realizados enxertos com fragmentos de pele colhidos do pescoco, assim como a aplicacao do PRP, preparado atraves de protocolo de dupla centrifugacao. As feridas com autoenxertos do lado esquerdo receberam PRP (grupo T), e as com autoenxertos do lado direito nao receberam tratamento (grupo C). Foram realizadas avaliacoes macroscopica e microscopica, considerando as variaveis integracao dos autoenxertos e retracao das bordas da ferida, alem de neovascularizacao, infiltrado inflamatorio, fibroblastos jovens, colagenizacao, reepitelizacao e integracao dos autoenxertos. Nao houve diferenca entre os grupos (p > 0,05) em relacao a maioria das variaveis macroscopicas e microscopicas. Contudo, a neovascularizacao foi significativamente maior (P = 0,0191) no grupo T, no 14o dia apos a realizacao da enxertia. Conclui-se que o PRP favorece o processo de reparo da pele com autoenxertos em equinos, ja que aumenta a neovascularizacao na fase inicial da cicatrizacao da ferida. Ainda, o PRP parece influenciar positivamente a integracao dos autoenxertos de pele e a retracao das bordas da ferida