Methodological study on rapid detection of heat-resistant mould-Byssochlamys nivea by PCR assay

According to the sequence of beta-tubulin of Byssochlamys nivea published in Genbank,two pairs of primers were designed.After PCR screening,the primer Bn-1 was selected to establish a PCR method for the detection of Byssochlamys nivea in this work.Futhermore,factors affecting PCR including annealing temperature and primer concentrations were optimized.Annealing temperature of 59 ℃ and primer concentrations of 0.2 μM/PCR system were optimal conditions.The specificity and sensitivity of PCR were also analyzed.The detection limit of genomic DNA in the PCR assay was 1 ng/PCR system.The established method provides a better choice for rapid detection of Byssochlamys nivea.