Construction and Analysis of Genomic Library of Bacillus licheniformis

As an efficient,innoxious,and biodegradable wastewater treatment agent with no secondary pollution,bioflocculant is receiving more and more concerns nowadays.In this paper,Bacillus licheniformis with excellent flocculating activity was used to construct the genomic library and tried to screen out the positive clones with flocculation gene.The results showed that the genomic library was successfully constructed,2.1×103 clones were screened out.17 clones were randomly selected for DNA sequencing and analysis.Some relative conserved proteins and 2 novel genes with unknown functions were found.The results lay a foundation for the further study of gene encoding bacteria flocculant and the whole genomic structure and function of Bacillus licheniformis.