HPLC Fingerprints of Nerviliae fordii and Nervilia plicata

OBJECTIVE: To establish the HPLC fingerprint of the whole plant of Nerviliae fordii and Nervilia plicata, in order to provide a method for evaluation of authenticity and quality control of the whole plant of Nerviliae fordii. METHODS: 15 batches of Nerviliae fordii and 5 batches of Nervilia plicata were analyzed on a Kromasil C18 column (250 mm x 4.6 mm, 5 μm) at room temperature with gradient elution using acetonitrile and 0.2% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The wavelength of detector was 256 nm. RESULTS: Significant differences were found between Nerviliae fordii and Nervilia plicata. Furthermore, there were no obvious differences observed between Nerviliae fordii of big-leaf and Nerviliae fordii of small-leaf. CONCLUSION: The present-developed HPLC fingerprints method provides a rapid, effective and valuable benchmark for distinguishing of Nerviliae fordii and Nervilia plicata, which is favorable to improve overall quality control of Nerviliae Fordii.