Inverse PCR amplification of low-abundancy message of γδ T cell receptor genes☆

The technique of inverse PCR permits the rapid amplification and identification of unknown DNA segments adjacent to well characterized core regions. In the field of immunology anchored PCR and inverse PCR are useful methods for examining junctional diversity and unknown variable gene segments of rearranged T cell receptor genes. We have applied an improved inverse PCR protocol to study the repertoire of γδ T cell receptor genes in the developing thymus of the mouse.