Molecular cloning and sequence analysis of phenylalanine ammonia-lyase cDNA from Pinus massoniana

Phenylalanine ammonia-lyase(PAL) plays a crucial role in phenylpropanoid metabolism and provides precursors of various phenylpropanoid compounds.According to the conservative domain of some other plants DNA sequences of PAL gene,a pair of degenerate primers was designed.A special DNA fragment was obtained by PCR amplifying,which length is 864 bp and it encoded a 288 amino acid protein.We analysed the nucleotide and amino of similarity to the corresponding gene of other organism,and the results show that it is a partial PAL gene of Pinus massoniana.The sequence was enrolled in the GenBank of NCBI.The accession number is GQ142010.