Study on the effect of paeoniae radix 801 on the interaction between interleukin-6 and its receptor

The immobilized amounts of interleukin-6 soluble receptor(sIL-6R) on the surface of quartz crystal microbalance(QCM) substrate are 297.08 ng·cm-2.interleukin-6(IL-6) can specifically bind to sIL-6R and the bound amounts are 33.70 ng·cm-2.The response for IL-6 is dose-dependent.Based on the analysis,the value for kass is 1.27×104(mol/L)-1s-1,the value for kdiss is 1.94×10-4s-1,the value for KD is 1.53×10-8 mol/L.Paeoniae radix 801 can enhance the binding amounts of IL-6/sIL-6R,which is dose-dependent.IL-6/sIL-6R is another action target of Paeoniae Radix 801 in vivo.