Quantification of Human Plasma-Busulfan Concentration by Liquid Chromatography-Tandem

Busulfan was separated and detected using an LC system containing a C18 column equipped with MS/MS. The sample was eluted with a mobile phase gradient for a total run time of 10 min. Plasma busulfan concentration was quantified against a 6-point standard curve in a multiple reaction monitoring mode at mass-to-charge (m/z) 264.1 > 151.1. Precision, recovery, matrix effect, linearity, detection capability, carryover, and sta-bility were evaluated. The range of plasma busulfan concentration was obtained by ana-lyzing samples from 9 children receiving busulfan.