Isolation, Purification and Identification of CFP29 from Mycobacterium tuberculosis H37Rv culture filtrate proteins

Tuberculosis (TB) continues to be a serious worldwide health issue. Human tuberculosis (TB) is the commonest cause of mortality from one infectious agent, with eight million new cases and two million fatalities each year. In many animal models of TB, proteins isolated from the culture filtrate of Mycobacterium tuberculosis promote protective immunity. The extracellular proteins of Mycobacterium tuberculosis were isolated, purified and identifiedin the current study. M. tuberculosis H37Rv was culturedin Souton’s medium and the extracellular proteins were isolated employing an ion-exchange column chromatography before their purification and characterization using SDS- PAGE, western blotting and N- terminal sequencing. CFP29 was identified and purified in M. tuberculosis H37Rv culture filtrate. However, further characterization of this protein is needed to be utilized as an effective T-cell antigen produced from culture filtrates.