967. Transgenic Genomic Reporter Assays for Functional Studies and Stem Cell Therapy

The development of Genomic Reporters Assays (GRAs), in which a reporter fusion is made to a gene in the context of its entire genomic locus on a Bacterial Artificial Chromosome (BAC) clone, preserves the normal location and spacing of many cis-acting regulatory elements that may be positioned over large distances in the surrounding chromosomal region, and facilitate the recapitulation of normal gene expression patterns. The generation of transgenic mice containing human genomic reporters readily facilitates the evaluation of developmental and tissue specific aspects of human gene expression in a whole animal model.