Practical uses of acridine orange fluorescence microscopy of centrifuged blood (QBC Malaria Test) and the QBCII Hematology System in patients attending malaria clinics in Thailand.

We evaluated the detection of malaria parasites using acridine orange fluorescence microscopy of centrifuged blood (AOFM/CB or «QBC Malaria Test TM ») at two government malaria clinics in rural Thailand. In a subgroup of the patients, a QBC Hematology Sytem TM for the determination of complete blood counts was also utilized. A Giemsa-stained thick smear (GTS) reading of 100 (1,000x) microscopic fields was used as standard. The AOFM/CB sensitivities were 97% overall and 95% for P. falciparum (Pf). Sensitivity was lower for P. vivax (Pv) (76%). Pv sensitivity depended largely on ameboid form density