Reversible electroporation on a microchip

The efficiency of electroporation for the porcine aortic endothelial valve cells is studied using pulsed dc electric fields and a new microchip applicator. The results indicate that maximum electroporation efficiency of over 80% can be achieved by choosing a proper amplitude and number of pulses. The result shows that increased field strength provides higher electroporation efficiency and maintains high cell viability within a limited value of applied electric charge. Our study also shows that the electroporation is reversible when the electric field is within 1 kV/cm.