Suppression subtractive hybridization to enrich low-abundance and submergence-enhanced transcripts of adventitious root primordia of Sesbania rostrata

Upon waterlogging, the tropical legume Sesbania rostrata activates dormant meristems of stem-located root primordia, leading to adventitious root outgrowth. By suppression subtractive hybridization (SSH), cDNA clones were collected that were enriched for low-abundant and water-induced transcripts. From 192 sequenced SSH clones, ranging from 64 to 1064 bp in size, 66 unigenes were obtained. Complex cDNA probes were generated to screen cDNA macro-arrays. Sequence homologies of putative up-regulated unigenes and of non-induced unigenes will be briefly discussed.