Method for improving phosphorus absorption efficiency of wheat plant by using sucrose transporter gene

The present invention discloses a method of using a plant sucrose transporter gene to improve the efficiency of phosphorus uptake wheat, comprising the steps of: A, cloned cDNA in TaSUT2; B, overexpression vector: B-1, cut with the restriction are enzymes SmaI and SacI digested intermediate vector plasmid pTSUT4 and monocotyledonous expression vector with pAHC25, be recovered after gel electrophoresis, recovered TaSUT2 1.9Kb fragment of pAHC25 and removing the GUS gene vector; B-2, the restriction on the after comprising TaSUT2 gene fragment encoding wheat sucrose transporter is connected to the carrier pAHC25: C, transgenic wheat obtained; TaSUT2 gene fragment was ligated to pAHC25 vector; B-3, will be transformed into E. coli DH5α competent cells connected system , transgenic methods using a gene gun mediated obtain transgenic wheat plants. The present invention TaSUT2 gene overexpression, transgenic wheat appears to improve the absorption efficiency of phosphorus and phosphorus enhanced phenotypic response explore new features the application direction of the gene.