Expression of the coat protein gene of IHHNV in shrimp in Pichia Pastoris

The infectious hypodermal haematopoietic necrosis virus is one of the main diseases of the penaeid shrimp.The gene of coat protein of IHHNV was constructed into Pichia Pastoris secretory expression vectors pPIC9K,named pPIC9K-IV.The recombinant plasmid pPIC9K-IV was linearized with BglⅡ,and then was transformed into Pichia Pastoris GS115 by electroporation.After PCR analysis and G418 screening,the pPIC9KIV recombinant was expressed with 0.8%(V/V) methanol.The expression product was confirmed by Western blot and ELISA analysis.The results showed that the coat protein was successfully expressed,its product was about 40ku,and could recognized specifically by rabbit polyclonal anti-serum.