A new method based on electron diffraction for detecting nanoparticles in injectable medicines

Abstract A new methods for detecting and characterize nanoparticles in an injectable pharmaceutical solution is presented. The methods is based on the simultaneous use, on those nanoparticles that are crystalline, of electron diffraction tomography and energy dispersive x-ray spectrometry (EDS). With electron diffraction tomography the unit cell and the crystal symmetry of the nanoparticles are determined, while with EDS the chemical composition is derived. With these data through an inspection of a crystallographic database it is possible to determine the crystal phase of the nanoparticles. The knowledge of the crystal phase is a valuable element for understanding the provenance and the formation of the nanoparticles, helping the researcher in solving any quality control issue related to the presence of nanoparticles in an injectable solution.