Characterization of the size-fractionated biomacromolecules: Tracking their role and fate in a membrane bioreactor

Abstract This article presents a study aimed at the fractionation and characterization of what is thought to be one of the most complex organic mixtures produced by activated sludge: biomacromolecules (BMM). Photometric quantification combined with excitation–emission matrix (EEM) fluorescence spectroscopy and nuclear magnetic resonance (NMR) measurements were used to characterize BMM in a membrane bioreactor (MBR) from a chemical perspective. Overall, the BMM in sludge supernatant were mainly present in three fractions: colloidal BMM (BMM c , >0.45 μm), biopolymeric BMM (BMM b , 0.45 μm–100 kDa) and low molecular weight (MW) fraction ( c fraction had similar carbon content of proteins and polysaccharides. In contrast, the BMM b and the low-MW BMM were proved to be carbonaceous and aromatics, respectively. Moreover, because of the high MW and gelling property, polysaccharides were found to have a high potential to accumulate on the membranes. In addition, the lipids present in the BMM b of the sludge supernatant were demonstrated to be another important foulant due to their large size. Our results also indicated that aromatic proteins had a higher fouling propensity than tryptophan proteins though they were of similar size nature. This work could be useful for better understanding of the chemical nature of BMMs in MBRs.