Standardization and HPTLC. Fingerprinting study of Poly Herbal Unani Formulation – Habb-e-Sara Khas

Standardization is used to describe all measures under taken during the manufacturing process and quality control of drug assuring its reproducible quality. Most of the traditional medicine are effective but still they lack in its standard parameters. Therefore, we need to develop standard techniques to standardize and validate herbal formulations. The drug Habb-e-Sara Khas is therapeutically useful in the treatment of Sara Khas (Epilepsy and Infantile epilepsy). The drug Habb-e-Sara Khas was prepared in three different batches as per the guidelines of National Formulary of Unani Medicine(Part-VI), Present study is aimed to evaluate the pharmacopoeial standards using physico-chemical parameters; HPTLC fingerprints, quality control and assurance parameters, using WHO guideline to ascertain the quality of drug. The physico-chemical data showed that the drug contain moisture(1.89%), total ash (5.43%),acid in-soluble(1.06%), alcohol and water soluble extractive matter (19.39%) and(60.34%),pH(1% solution) (5.93),pH(10% solution)(5.67), ASSE(18.07%) and CSSE (17.43%), Bulk density of granules(0.4989) and the TLC/HPTLC finger prints showed various spots at 254nm, 366nm and visible light (V-S reagent). The quality control study revealed the absence of microbial load, aflatoxins, heavy metals and pesticide residues, The evaluated standards will very much useful for laying the phamacopoeial standards of Habb-e-Sara Khas and also in providing the quality medicine to needful human beings.