Isolation of T lymphocyte subsets from peripheral blood using monoclonal antilymphocyte antibodies

M onoclonal an tise ra against h e lp e r (serum OKT4) and su p p resso r (serum OKT8) T cells were em ployed in a com plem ent-dependent lymphocytotoxicity reaction to isolate he lper and suppressor T cells from p eriphera l blood m ononuclear cells. W hen p repared by this m ethod, he lper and suppressor T cells had a purity of 67.8 percent and 78.8 pe r­ cent, respectively. Approximately 56 percent of viable suppressor and 33 percen t of viable helper T cells were lost during the procedure.