Experimental study of scalp-acupuncture on the express of GLUT1 after rat intracerebral hemorrhage

Objective:To prove the effect of acupuncture through an experimental study of scalp-acupuncture on the express of GLUT1 after intracerebral hemorrhage in rats.Method:Wistar rats were divided into 4 groups:normal group,sham group,model group,acupuncture group.The last three group was divided into three time groups(24h,72h,168h) ,The model of intracerebral hemorrhage in rat was made with Collagenase Ⅶ/heparini infusion,and treated with electro-acupuncture of Baihui-Taiyang points,Immunohistochemical method used to observe the expression of GLUT1 around the hemotoma.Result:The model group after intracerebral hemorrhage:morphology of majority positive GLUT1 capillary vessels showed narrowing,shrinking and obliteration in three time points specially near the focus of lesion.The expression of GLUT1 in 24h revealed a compensatory rising at first,compared with control group(P0.05) ;then lowered,in 72h and in 168h(P0.05) ;Acupuncture group:in perihemotoma area,the morphology of positive GLUT1 capillary vessels showed slightly narrowing only in 24h,then most of the vessels showed spherical distending,GLUT1 decreased in 72h and 168h(P0.05) ,but better than the model group(P0.05) .Conclusion:Acupuncture have therapeutic effects on three time points,delay the development of pathologic change in time,lengthen the therapy time window,and obviously effect in the earlier period.