Cytological diagnosis and therapeutic management of lymphosarcoma in a Murrah buffalo: A clinical study

A six year old non-pregnant female buffalo was presented to Teaching veterinary Polyclinics, International Institute of Veterinary Education and Research (IIVER) Rohtak, Haryana with history of difficulty in mastication, intermittent inappetance, sudden swelling on the right side of face including mandibular and parotid region which extended on the left side of the face from last 15 days. On cytological examination, the case was diagnosed as lymphosarcoma based on presence of abundant number of pleomorphic lymphocytes in the pre-scapular lymph node aspirate. Treatment with a single dose of Vincristine sulphate @ 0.02 mg/kg body weight in one litre normal saline intravenously was done and advised for follow up of the case. There was a substantial improvement in the size of the affected lymph nodes along with moderate reduction in the number of lymphocytes on cytological examination of pre-scapular lymph node aspirate on day 10 post treatment. The animal was given a second dose of Vincristine sulphate at same dose rate and route to achieve complete cytological recovery, which was evident on day 21 post treatment.