Microfluidic cell array chip for high-throughput medicament screening, method and use

The invention relates to a microfluidic cell array chip for high-throughput medicament screening, a preparation method thereof and use thereof. The microfluidic cell array chip for high-throughput medicament screening is characterized by sequentially comprising a valve-controlled channel layer, a fluid channel layer and a glass layer suitable for the anchorage growth of cells from top down. The chip can be used in high-throughput medicament screening by the parallel action of different medicament concentrations on various cells. The chip is manufactured by a multixposure one-development Su-8 negative photoresist process and multilayer PDMS bonding and has a structure with a high depth-to-width ratio. The microfluidic cell array chip provided by the invention can realize low reagent consumption and high-throughput co-culture of various cells, parallel analysis of the stimulation effect of different medicament concentrations on different cells and the real-time observation and detection of a slide of the cells and provide a fire-new technical platform and a method for high-throughput medicament screening and cell-medicament researches.