Degradation of surface-active compounds in a constructed wetland determined using high performance liquid chromatography and extraction spectrophotometry

Degradation of anionic and nonionic surfactants in a constructed wetland with horizontal subsurface flow was studied using high performance liquid chromatography and extraction spectrophotometry. The ratio of individual homologues of linear alkylbenzene sulfonates (LAS) and the efficiency of their removal were studied. Tridecyl-, dodecyl-, undecyl-, and decylbenzene sulfonates were removed with efficiencies of 92.9%, 84.3%, 64.7%, and 41.1%, respectively. These differences are due to sequential shortening of the alkyl chain in homologues during degradation (the higher homologue can provide the lower one). The formation of sulfophenyl carboxylic acids during ω-oxidation of the alkyl chain followed by successive α- and/or β-oxidation is also a possible mechanism for removal of LAS. Solid phase extraction using Chromabond® HR-P columns was used for preconcentration of the analytes prior to their determination by HPLC. Methylene blue active compounds were determined using extraction spectrophotometry. The ave...