Purification, characterization and immunomodulatory activity of polysaccharides from Leccinum crocipodium (Letellier.) Watliag

Abstract The polysaccharide obtained from edible fungi has been regarded as the major bioactive component related to the nutritional and human's health. In the present study, three purified polysaccharides (LCP-1, LCP-2, and LCP-3) were obtained from Leccinum crocipodium (Letellier.) Watliag. The characterizations of LCP-1, LCP-2, and LCP-3 were determined by high-performance liquid chromatography (HPLC), UV, FT-IR spectrometrys and 1H NMR spectrum. LCP-1 had a molecular weight of 2.303 × 105 Da and 7.519 × 103 Da, and was composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (GluA), galacturonic acid (GalA), glucose (Glu), galactose (Gal), xylose (Xyl), arabinose (Ara) and fucuronic (Fuc). The molecular weight of LCP-2 was 2.655 × 105 Da, and its monosaccharide constituents were Man, Rib, Rha, GluA, Glu, Gal, Xyl, Ara and Fuc. The molecular weight of LCP-3 was 3.783 × 105 Da, and its monosaccharide constituents were Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Ara and Fuc. For the in vitro immunomodulatory experiments demonstrated that three purified polysaccharides could enhance immunomodulatory activities on macrophage RAW 264.7 cells, moreover, LCP-2 and LCP-3 showed stronger immunomodulatory activity than LCP-1. The results indicated that the LCP-1, LCP-2, and LCP-3 could be further developed as functional food or medicine.