Deterministic Lateral Displacement as a Function of Particle Size Using a Piecewise Curved Planar Interdigitated Electrode Array

Key Words: Blood(혈액), Cell Separation(세포분리), Interdigitated Electrode Array(교차전극배열), Lateral-Dielectrophoresis(측면유전영동), Microfluidics(미소유체)초록: 본논문에서는유체흐름방향과예각으로놓여있는평면교차전극위를지나가는입자의크기에따른측면방향변위에대하여소개한다. 아울러, 유체흐름방향과평면교차전극사이의각도에따른측면방향변위의변화를보인다. 본논문에서는선전하(linecharge) 모델을이용하여크기가다른세종류의형광polystyrene(PS) 입자의측면방향변위를이론적으로예측하였다. 크기에따른입자의측면방향변위의변화를이용하여, 크기별로입자를연속적으로분리할수있는측면방향유전영동미세분리기를개발하였다. 3, 5, 10μmPS입자의혼합물을이용하여분리한실험결과로부터개발된측면방향유전영동미세분리기는3 μm PS 입자를99.86%, 5 μm를98.82%, 10 μm를99.69%의높은효율로각각분리할수있음을확인하였다. 이로부터제안된측면방향유전영동미세분리기는다종혼합물로부터다양한크기의입자들을한번에분리하는기술로널리활용될수있을것으로기대된다.Abstract: This paper presents the lateral displacement of a particle passing over a planar interdigitated electrodearray at an angle as a function of particle size. The lateral displacement was also measured as a function of theangle between the electrode and the direction of flow. A simplified line charge model was used for numericalestimation of the lateral displacement of fluorescent polystyrene (PS) beads with three different diameters. Using thelateral displacement as a function of particle size, we developed a lateral dielectrophoretic (DEP) microseparator,which enables the continuous discrimination of particles by size. The experiment using an admixture of 3-, 5-, and10-μm PS beads showed that the lateral DEP microseparator could continuously separate out 99.86% of the 3-μmbeads, 98.82% of the 5-μm beads, and 99.69% of the 10-μm beads. The lateral DEP microseparator is thus apractical device for the simultaneous separation of particles according to size from a heterogeneous admixture.