The Hough Transform as a Basis for Image Recognition and Fluorescent Nanoparticle Tracking

A technique for image processing and the localization (tracking) of fluorescent nanoparticles is developed on the basis of the Hough transform. Features of data analysis in the Hough parametric space that are due to the need to display the coordinates of single point emitters in 3D space are discussed. Examples are given of tracking single point emitters in the form of colloidal semiconductor CdSe/ZnS nanocrystals.