Furin‐Mediated Self‐Assembly of Olsalazine Nanoparticles for Targeted Raman Imaging of Tumors

Olsalazine (Olsa) is a broad-spectrum anti-cancer agent acting as a DNA methylation inhibitor. When conjugated to 2-cyano-6-aminobenzothiazole and a peptide substrate specific for the tumor-overexpressed enzyme furin, it can self-assemble into nanoparticles that can be detected by chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).  We report here that these nano-assemblies can also be detected with high specificity in furin-overexpressing tumor cells by Raman spectroscopy with a distinct scattering signature and demonstrate the utility of this sensing mechanism in vitro and in vivo. Our findings suggest that Raman spectroscopy could be used for high-resolution image-guided surgery to precisely delineate tumor margins during and after resection in real-time as well as to determine microscopic tumor invasion and multifocal locoregional tumor spread, which are currently impossible to visualize with available imaging technologies including CEST MRI.