Fluorescence detection device

The invention provides a fluorescence detection device which comprises a detection pool, an emitting fiber, a focusing lens, a receiving fiber and a receiver, wherein the detection pool is cylindrical, a side wall of the detection pool is a transparent side wall, and an opening is formed at the upper end of the detection pool; the focusing lens is mounted above the opening of the detection pool; one end of the emitting fiber corresponds to a position above the focusing lens, and the other end of the emitting fiber is mounted on an excitation light source; the receiving fiber comprises a winding part and a transferring part, the winding part is provided with a fiber core, and the transferring part is provided with a fiber core and a coating layer; the winding part winds outside the side wall of the detection pool, and the tail end of the transferring part is coupled with the receiver. The fluorescence detection device provided by the invention can effectively improve the fluorescence collection efficiency, receive fluorescence from all dimensions and improve the detection precision, and has the advantages of small volume and facilitation of implementation.