Novel yeast isolated from broilers' feedstuff, gut and faeces as aflatoxin B1 adsorbents.

Aims isolate and characterize native yeast strains from broilers' environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results A total of 9 yeast strains were isolated: 3 from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), 2 from gut identified as Candida tropicalis and 4 from faeces identified as C. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out the adsorptions studies, one strain from each genus and each origin was selected as follows: C. lusitaniae and P. kudriavzevii from feedstuff, C. lusitaniae and Cyberlindnera fabianii from feaces and C. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107cell ml−1 and 100 ng ml−1 of AFB1, respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherms studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the complex AFB1 yeast demonstrated the irreversibility of the binding process. Conclusion Yeast strains tested in the present work constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study This work makes a contribution on using of native yeasts from broilers' environment for controlling chronic aflatoxicosis in avian production. This article is protected by copyright. All rights reserved.