Effects of flavonoids on parietal cell acid secretion, gastric mucosal prostaglandin production and Helicobacter pylori growth

The effect of the flavonoids flavone, flavanone and quercetin on parietal cell acid production, H + /K + -ATPase activity, gastric mucosal prostaglandin E 2 biosynthesis and Helicobacter pylori growth was studied. All flavonoids inhibited acid production in isolated parietal cells in response to histamine and dibutyryl-cAMP stimulation (IC 50 values between 26 and 139 pmol/l) and inhibited H + /K + -ATPase activity. Inhibition of H + /K + -ATPase activity was dependent on the ATP concentration. Fluorescence measurements showed that flavanone reacts with ATE These findings indicate that the inhibitory action of flavonoids on H + /K + -ATPase activity is related to their ability to complex ATP. Flavone and flavanone (10 and 100 μmol/l) stimulated prostaglandin E 2 production in isolated gastric mucosal cells. Furthermore, the compounds inhibited Helicobacter pylori growth in a concentration-dependent manner. From these finding it appears that flavonoids are a group of compounds which could have a therapeutic potential for treatment of gastrointestinal diseases associated with Helicobacter pylori infection.