Partial interdigitation of lipid bilayers
2011
A methodology has been developed to detect partial interdigitation of lipid bilayers when a bioactive molecule is intercalated between the polar, interface, or hydrophobic segments. This methodology uses the easily accessible differential scanning calorimetry (DSC) technique as a screening one and the increase of DH due to the incorporated drug in lipid bilayers as a diagnostic thermodynamic parameter. The combined use of X-ray diffraction and Raman spectroscopy complement and confirm the provided by DSC information as it is shown in three classes of molecules, namely AT1 antagonists, vinca alkaloids, and anesthetic steroids. For the two classes of molecules, AT1 antagonists and vinca alkaloids, their presence in lipid bilayers results in the increase of DH and it is accompanied by the increase of trans:gauche ratio and the decrease of d-spacing as depicted by Raman spectroscopy and small-angle X-ray diffraction correspondingly, confirming the predictive ability of DSC experiments. When an anesthetic steroid is incorporated in lipid bilayers, neither increase of DH nor decrease of d-spacing was observed, confirming again the DSC results that show the absence of partial interdigitation of this class of molecules. Molecular dynamics simulations have been carried out for a representative system ((5S)-1- benzylo-5-(1H-benzimidazol-1-ylo-methylo)-2-pyrrolidinone (MMK3) ligands at 1,2- dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayer), and the results confirmed the experimental findings. The change of distance at z-axis of oxygen atoms at head group of lipid molecule has been measured throughout the simulations. Statistical analysis has shown � 8.8 Ainterdigitation. Derived computational results are encouraging and can be performed
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