Target genes of hsa-miR-17-92 cluster predicted by bioinformatics analysis

Objective To analyze the prediction of target genes of hsa-miR-17-92 clusters and the paralogs, so as to lay foundation and provide theoretical basis for the further studies of hsa-miR-17-92 cluster biological function in human preadipocytes and obesity development. Methods 1.All literatures concerning miR-17-92 clusters were searched in PubMed and Google; 2.miRBase database was used to retrieve the sequence of hsa-miR-17-92 clusters according to the"seed sequence"to classify the member; 3.National Center for Biotechnology Information(NCBI) blast, NCBI mapviewer and UCSC Genome Browser were used to analyze the characteristics of hsa-miR-17-92 clusters and the paralogs; 4.TargetScan5.1, PicTar and miRanda were used to predict target genes of hsa-miR-17-92 clusters, and the intersection of the 3 results as gene set was analyzed by Gene Ontologe(GO) analysis and Pathway analysis. Results 1.hsa-miR-17-92 clusters and the paralogs had been reported in adipocyte differentiation, cancer, heart and lung develo-pment, the immune system and angiogenesis, and involved in other biological process as well; 2.hsa-miR-17-92 cluster members were highly conservative and divided into 4 categories; 3.the target genes set mostly existed in cell cycle, cell adhesion, Wnt signaling pathway, TGF-β signaling pathway, p53, mitogen-activated protein kinase(MAPK) and other signaling pathway and prostate cancer, pancreatic cancer and colon cancer and other diseases pathway. Conclusions The target genes set of hsa-miR-17-92 cluster enrich in multiple biological process for laying the foundation for the further study in human preadipocytes. Key words: hsa-miR-17-92 cluster; Bioinformatics analysis; Human preadipocytes; Obesity