含14-3-3蛋白抑制肽R18重组腺病毒制备及其在心肌细胞中的表达

Objective: To construct a recombinant adenovirus expressing R18, an inhibitory peptide of 14-3-3 protein. Methods: The adenovirus expressing R18 (AdR18) was prepared by the homorecomnination method, then identified and amplified. The high titer adnovirus product was used to infect neonatal cardiac myocyte, and the expression of R18 in cardiac myocyte was detected by PCR and Western blot. Results: 48 hours after infected, the expression of R18 was detected in the AdR18 transfected group by western blot assay, but it was not found in the control Ad group. Conclusion: Adenovirus can high efficiently induce the expression of exogene in cardiac myocyte.