Regulation of the synthesis and assembly of the plant vacuolar H{sup +}-ATPase. Progress report, [April 1, 1991--March 31, 1992]

During the past three years we have focused on four main areas: the characterization of the 5{prime}-upstream sequence of the gene for the V-ATPase 70 kDa (A) subunit gene, the generation of V-ATPase-deficient mutants using antisense constructs of the A subunit cDNA, analysis of V-ATPase ultrastructure by negative staining and the characterization of organelle-specific isoforms of the A subunit of carrot. In addition we have extended our studies on the cellular distribution of the V-ATPase and we have continued our investigation of the evolution of the V-ATPases by characterizing the A and B subunits of two species of the archaebacterium, Methanococcus.