Plant pollen tube cell calcium ion ultrastructure positioning method

The invention discloses a plant pollen tube cell calcium ion ultrastructure positioning method which mainly comprises the following steps: (1) preparing a quantitative immobilizing liquid and washing liquid; (2) immobilizing and washing a material; (3) immobilizing and washing secondarily; (4) dehydrating and transiting; (5) carrying out resin permeating, embedding and polymerizing; (6) directly observing by using an electron microscope without dyeing the material. Due to the adoption of the method, the problems that cell turgor pressure needs to be adjusted, abnormal reaction can be caused between potassium pyroantimonate and buffer liquid, calcium pyroantimonate precipitation displacement or dilution can be caused by material immobilization and aftertreatment, Ca precipitate observation can be affected because of inappropriate dyeing, and the like can be solved, and organelle can be clearly distinguished without dyeing completely.