Method for cultivating cucumber breeding material by utilizing macrospore culture technique

2013 
The invention provides a method for cultivating a cucumber breeding material by utilizing a macrospore culture technique. The method for cultivating the cucumber breeding material by utilizing the macrospore culture technique comprises the steps of selecting ovaries of cucumbers, sterilizing, slicing, carrying out high temperature induction treatment, carrying out subculture, and carrying out plant regeneration. By adopting the method for cultivating the cucumber breeding material by utilizing the macrospore culture technique, the plant regeneration rate can be 20%, and the ratio of dihaploid plants obtained by virtue of naturally doubling in regenerated plants is 80%. The method for cultivating the cucumber breeding material by utilizing the macrospore culture technique is applicable to main planting varieties, such as spotted cucumbers and dry cucumbers, in China and is also applicable to cucumbers in Japan and Europe, the breeding material can be obtained through screening in a field, and the breeding material is applied to breeding of cucumber varieties, so that the breeding process is sped up.
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